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wheat germ agglutinin wga  (Vector Laboratories)


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    Structured Review

    Vector Laboratories wheat germ agglutinin wga
    Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1025-5/Biotinylated+Wheat+Germ+Agglutinin+(WGA)/pmc12973475-82-39-43
    Average 94 stars, based on 272 article reviews
    wheat germ agglutinin wga - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Whole Genome Amplification:

    Article Title: Butyrate Rescues Cardiac Metabolic Dysfunction in Hypertensive Heart Failure with Preserved Ejection Fraction
    Article Snippet: .. Primary antibody (Rabbit anti-rat Fibronectin antibody [1:75] Ab23751; Abcam, Mouse anti-rat CD68 antibody | Clone ED1 [1:50] MCA341R; Bio-Rad) or biotinylated wheat germ agglutinin (WGA [1:100] B1025 VectorLab) or sirius red solution (0.1% in picric acid, Sigma) was diluted in 10% NDS and applied to the slice. .. After incubation in a humidity chamber overnight (4°C) or 60 min (Sirius red), excess antibody was removed, Cy3-conjugated secondary antibody (715-165-151, 711-165-152, respectively; Jackson ImmunoResearch) was diluted 1:100 or 1:300 in 1x PBS and incubated in a humidity chamber (120 min at RT).

    Article Title: Fluid shear stress-dependent modulation of the basal endothelial glycocalyx
    Article Snippet: .. Samples were allowed to incubate in primary solution containing biotinylated wheat germ agglutinin (WGA) (1:500, Vector) in 1% BSA in PBS overnight. ..

    Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
    Article Snippet: .. The membranes were blocked for nonspecific binding with 5% bovine serum albumin (w/v) in TBST for 1 h at room temperature, and then incubated with 0.25 μg/mL biotinylated lectins as follows; concanavalin A (Con A) (Vector Laboratories, Newark, NJ), wheat germ agglutinin (WGA) (Vector Laboratories), and Phaseolus Vulgaris erythroagglutinin (PHA-E) (Vector Laboratories), for 1 h at room temperature. .. The membranes were washed with TBST for 10 min, three times, and incubated with streptavidin-horseradish peroxidase conjugate (Invitrogen, Frederick, MD) for 1 h at room temperature.

    Binding Assay:

    Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
    Article Snippet: .. The membranes were blocked for nonspecific binding with 5% bovine serum albumin (w/v) in TBST for 1 h at room temperature, and then incubated with 0.25 μg/mL biotinylated lectins as follows; concanavalin A (Con A) (Vector Laboratories, Newark, NJ), wheat germ agglutinin (WGA) (Vector Laboratories), and Phaseolus Vulgaris erythroagglutinin (PHA-E) (Vector Laboratories), for 1 h at room temperature. .. The membranes were washed with TBST for 10 min, three times, and incubated with streptavidin-horseradish peroxidase conjugate (Invitrogen, Frederick, MD) for 1 h at room temperature.

    Incubation:

    Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
    Article Snippet: .. The membranes were blocked for nonspecific binding with 5% bovine serum albumin (w/v) in TBST for 1 h at room temperature, and then incubated with 0.25 μg/mL biotinylated lectins as follows; concanavalin A (Con A) (Vector Laboratories, Newark, NJ), wheat germ agglutinin (WGA) (Vector Laboratories), and Phaseolus Vulgaris erythroagglutinin (PHA-E) (Vector Laboratories), for 1 h at room temperature. .. The membranes were washed with TBST for 10 min, three times, and incubated with streptavidin-horseradish peroxidase conjugate (Invitrogen, Frederick, MD) for 1 h at room temperature.

    Article Title: Proteins having pneumococcal capsule degrading activity and methods of use
    Article Snippet: .. For microscopy, cells were incubated at room temperature for 30 minutes with a 1/500 dilution of biotinylated wheat germ agglutinin (Vector labs) in 1% bovine serum albumin followed by a 30-minute room temperature incubation with a 1/1000 dilution of streptavidin APC (Biolegend). .. 107 bacteria were added to a confluent monolayer of murine leukemia virus transformed macrophage line, RAW 264.7 (American Type Culture Collection (ATCC) Manassas, VA) with active or heat-inactivated baby rabbit complement (Pel-Freez) in a 24 well plate and incubated for 1 hour at 37° C. Wells were washed 4× with PBS to remove extracellular bacteria.

    Microscopy:

    Article Title: Proteins having pneumococcal capsule degrading activity and methods of use
    Article Snippet: .. For microscopy, cells were incubated at room temperature for 30 minutes with a 1/500 dilution of biotinylated wheat germ agglutinin (Vector labs) in 1% bovine serum albumin followed by a 30-minute room temperature incubation with a 1/1000 dilution of streptavidin APC (Biolegend). .. 107 bacteria were added to a confluent monolayer of murine leukemia virus transformed macrophage line, RAW 264.7 (American Type Culture Collection (ATCC) Manassas, VA) with active or heat-inactivated baby rabbit complement (Pel-Freez) in a 24 well plate and incubated for 1 hour at 37° C. Wells were washed 4× with PBS to remove extracellular bacteria.

    Avidin-Biotin Assay:

    Article Title: Profiling of peptides from wet- and dry-heated milk proteins after in vitro infant digestion and intestinal transport, and their effects on mucus production and innate immunity.
    Article Snippet: QPCR primers were purchased from Biolegio BV. .. Biotinylated wheat germ agglutinin (B-1025) and avidin-peroxidase conjugate from the VECTASTAIN® Elite® ABC-HRP Kit (PK-6100) were obtained from Vector Laboratories, and sulfuric acid was obtained from Brunschwig. .. Porcine pepsin (P6887), porcine pancreatin (P7545), porcine bile extract (B8631), Dulbecco’s Phosphate buffered saline (DPBS) with and without CaCl2 +/MgCl2+, prostaglandin E2 (PGE2), mucin from porcine stomach (M1778), bovine serum albumin (BSA; A3059), o-phenylenediamine dihydrochloride (P8787), Alcian Blue 8GX (A5268), PAS staining kit (1.1646.0001) and all other chemicals were obtained from Sigma Aldrich.



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    ONX-0914 inhibits prostate cancer progression by promoting TCF7L1 O-GlcNAcylation and subsequently suppressing AR expression. ( A ) Venn diagram showing 13 overlapping AR-associated transcription factors identified from bioinformatic analyses integrating JASPAR, TCGA, and GEO datasets comparing AR-high vs. AR-low prostate cancer samples. ( B ) AR expression changes of candidate transcription factors in LNCaP cells treated with ONX-0914, based on qPCR screening. ( C ) qPCR analyses showing that AR expression is suppressed following TCF7L1 overexpression (OE TCF7L1) and upregulated following TCF7L1 knockdown (si TCF7L1). ( D ) Immunoblot analysis confirming AR expression is suppressed following OE TCF7L1 and upregulated following si TCF7L1. ( E ) Immunoblot analysis showing that TCF7L1 upregulation by ONX-0914 is reversed by DON. ( F ) Co-immunoprecipitation (Co-IP) using anti-HA antibody in HA-tagged TCF7L1-overexpressing cells reveals O-GlcNAcylation of TCF7L1. ( G ) <t>sWGA</t> pull-down confirms that TCF7L1 is O-GlcNAc modified; modification is enhanced by PUGNAc and suppressed by OSMI-1. ( H , I ) Co-IP assays performed with anti-HA ( H ) or anti-Flag ( I ) in HA-TCF7L1/Flag-OGT co-transfected cells, demonstrating their interaction. ( J ) Co-IP shows native TCF7L1 also interacts with endogenous OGT in LNCaP cells. ( K ) Co-IP analysis shows ONX-0914 increases TCF7L1 O-GlcNAcylation. ( L ) sWGA pull-down further validates that ONX-0914 promotes O-GlcNAcylation of TCF7L1 in LNCaP cells. ( M ) Cell colony images showing reduced colonies density and proliferation in the OE TCF7L1 group compared to NC and siTCF7L1. ( N ) Quantification of cell colonies in each group. ( O ) CCK-8 assay demonstrates suppressed proliferation following TCF7L1 overexpression. ( P , Q ) Transwell assays and quantification indicate significantly reduced migration and invasion in the OE TCF7L1 group compared to controls. ( R , S ) qPCR ( R ) profiling of EMT-related transcripts and immunoblot ( S ) detection of EMT-associated proteins in NC, OE TCF7L1, and si TCF7L1 groups.
    Biotinylated Swga Magnetic Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ONX-0914 inhibits prostate cancer progression by promoting TCF7L1 O-GlcNAcylation and subsequently suppressing AR expression. ( A ) Venn diagram showing 13 overlapping AR-associated transcription factors identified from bioinformatic analyses integrating JASPAR, TCGA, and GEO datasets comparing AR-high vs. AR-low prostate cancer samples. ( B ) AR expression changes of candidate transcription factors in LNCaP cells treated with ONX-0914, based on qPCR screening. ( C ) qPCR analyses showing that AR expression is suppressed following TCF7L1 overexpression (OE TCF7L1) and upregulated following TCF7L1 knockdown (si TCF7L1). ( D ) Immunoblot analysis confirming AR expression is suppressed following OE TCF7L1 and upregulated following si TCF7L1. ( E ) Immunoblot analysis showing that TCF7L1 upregulation by ONX-0914 is reversed by DON. ( F ) Co-immunoprecipitation (Co-IP) using anti-HA antibody in HA-tagged TCF7L1-overexpressing cells reveals O-GlcNAcylation of TCF7L1. ( G ) <t>sWGA</t> pull-down confirms that TCF7L1 is O-GlcNAc modified; modification is enhanced by PUGNAc and suppressed by OSMI-1. ( H , I ) Co-IP assays performed with anti-HA ( H ) or anti-Flag ( I ) in HA-TCF7L1/Flag-OGT co-transfected cells, demonstrating their interaction. ( J ) Co-IP shows native TCF7L1 also interacts with endogenous OGT in LNCaP cells. ( K ) Co-IP analysis shows ONX-0914 increases TCF7L1 O-GlcNAcylation. ( L ) sWGA pull-down further validates that ONX-0914 promotes O-GlcNAcylation of TCF7L1 in LNCaP cells. ( M ) Cell colony images showing reduced colonies density and proliferation in the OE TCF7L1 group compared to NC and siTCF7L1. ( N ) Quantification of cell colonies in each group. ( O ) CCK-8 assay demonstrates suppressed proliferation following TCF7L1 overexpression. ( P , Q ) Transwell assays and quantification indicate significantly reduced migration and invasion in the OE TCF7L1 group compared to controls. ( R , S ) qPCR ( R ) profiling of EMT-related transcripts and immunoblot ( S ) detection of EMT-associated proteins in NC, OE TCF7L1, and si TCF7L1 groups.
    Biotinylated Wheat Germ Agglutinin (Wga), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1025-5/Biotinylated+Wheat+Germ+Agglutinin+(WGA)/custom%40b-1025%4041349547
    Average 94 stars, based on 1 article reviews
    biotinylated wheat germ agglutinin (wga) - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    ONX-0914 inhibits prostate cancer progression by promoting TCF7L1 O-GlcNAcylation and subsequently suppressing AR expression. ( A ) Venn diagram showing 13 overlapping AR-associated transcription factors identified from bioinformatic analyses integrating JASPAR, TCGA, and GEO datasets comparing AR-high vs. AR-low prostate cancer samples. ( B ) AR expression changes of candidate transcription factors in LNCaP cells treated with ONX-0914, based on qPCR screening. ( C ) qPCR analyses showing that AR expression is suppressed following TCF7L1 overexpression (OE TCF7L1) and upregulated following TCF7L1 knockdown (si TCF7L1). ( D ) Immunoblot analysis confirming AR expression is suppressed following OE TCF7L1 and upregulated following si TCF7L1. ( E ) Immunoblot analysis showing that TCF7L1 upregulation by ONX-0914 is reversed by DON. ( F ) Co-immunoprecipitation (Co-IP) using anti-HA antibody in HA-tagged TCF7L1-overexpressing cells reveals O-GlcNAcylation of TCF7L1. ( G ) sWGA pull-down confirms that TCF7L1 is O-GlcNAc modified; modification is enhanced by PUGNAc and suppressed by OSMI-1. ( H , I ) Co-IP assays performed with anti-HA ( H ) or anti-Flag ( I ) in HA-TCF7L1/Flag-OGT co-transfected cells, demonstrating their interaction. ( J ) Co-IP shows native TCF7L1 also interacts with endogenous OGT in LNCaP cells. ( K ) Co-IP analysis shows ONX-0914 increases TCF7L1 O-GlcNAcylation. ( L ) sWGA pull-down further validates that ONX-0914 promotes O-GlcNAcylation of TCF7L1 in LNCaP cells. ( M ) Cell colony images showing reduced colonies density and proliferation in the OE TCF7L1 group compared to NC and siTCF7L1. ( N ) Quantification of cell colonies in each group. ( O ) CCK-8 assay demonstrates suppressed proliferation following TCF7L1 overexpression. ( P , Q ) Transwell assays and quantification indicate significantly reduced migration and invasion in the OE TCF7L1 group compared to controls. ( R , S ) qPCR ( R ) profiling of EMT-related transcripts and immunoblot ( S ) detection of EMT-associated proteins in NC, OE TCF7L1, and si TCF7L1 groups.

    Journal: Oncology Research

    Article Title: ONX-0914 Suppresses Hormone-Sensitive Prostate Cancer by Promoting O-GlcNAcylation-Mediated Stabilization of TCF7L1

    doi: 10.32604/or.2026.073156

    Figure Lengend Snippet: ONX-0914 inhibits prostate cancer progression by promoting TCF7L1 O-GlcNAcylation and subsequently suppressing AR expression. ( A ) Venn diagram showing 13 overlapping AR-associated transcription factors identified from bioinformatic analyses integrating JASPAR, TCGA, and GEO datasets comparing AR-high vs. AR-low prostate cancer samples. ( B ) AR expression changes of candidate transcription factors in LNCaP cells treated with ONX-0914, based on qPCR screening. ( C ) qPCR analyses showing that AR expression is suppressed following TCF7L1 overexpression (OE TCF7L1) and upregulated following TCF7L1 knockdown (si TCF7L1). ( D ) Immunoblot analysis confirming AR expression is suppressed following OE TCF7L1 and upregulated following si TCF7L1. ( E ) Immunoblot analysis showing that TCF7L1 upregulation by ONX-0914 is reversed by DON. ( F ) Co-immunoprecipitation (Co-IP) using anti-HA antibody in HA-tagged TCF7L1-overexpressing cells reveals O-GlcNAcylation of TCF7L1. ( G ) sWGA pull-down confirms that TCF7L1 is O-GlcNAc modified; modification is enhanced by PUGNAc and suppressed by OSMI-1. ( H , I ) Co-IP assays performed with anti-HA ( H ) or anti-Flag ( I ) in HA-TCF7L1/Flag-OGT co-transfected cells, demonstrating their interaction. ( J ) Co-IP shows native TCF7L1 also interacts with endogenous OGT in LNCaP cells. ( K ) Co-IP analysis shows ONX-0914 increases TCF7L1 O-GlcNAcylation. ( L ) sWGA pull-down further validates that ONX-0914 promotes O-GlcNAcylation of TCF7L1 in LNCaP cells. ( M ) Cell colony images showing reduced colonies density and proliferation in the OE TCF7L1 group compared to NC and siTCF7L1. ( N ) Quantification of cell colonies in each group. ( O ) CCK-8 assay demonstrates suppressed proliferation following TCF7L1 overexpression. ( P , Q ) Transwell assays and quantification indicate significantly reduced migration and invasion in the OE TCF7L1 group compared to controls. ( R , S ) qPCR ( R ) profiling of EMT-related transcripts and immunoblot ( S ) detection of EMT-associated proteins in NC, OE TCF7L1, and si TCF7L1 groups.

    Article Snippet: The treated samples were incubated overnight at 4°C with biotinylated sWGA magnetic beads (Vector Laboratories, B-1025S-5, Newark, CA, USA).

    Techniques: Expressing, Over Expression, Knockdown, Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Modification, Transfection, CCK-8 Assay, Migration